Plant Tissue Culture Concepts and Laboratory Exercises furnishes students, instructors, and researchers with a broad consideration of the field. It provides historical perspectives, state-of-the-art techniques and methods, along with future advances and applications. TOC:From the contents: Introduction to plant tissue culture.- History of plant tissue and cell culture.- Nutrition of callus cultures.- Histological techniques.- Photographic methods for plant cell and tissue culture.- Statistical analysis of plant tissue culture data.- Propagation from preexisting meristems.- Micropropagation of syngonium by shoot culture.- Micropropagation of Dieffenbachia.- Micropropagation of potato by node culture and microtuber production.- Micropropagation of lilacs.- Micropropagation and in vitro flowering of rose.- Propagation from nonmeristematic tissues organogenesis.- Direct shoot organogenesis from leaf explants of chrysanthemum.- Shoot organogenesis from cotyledon explants of watermelon.- Direct and indirect shoot organogenesis from leaves of Torenia fournier.- Shoot organogenesis from petunia leaves.- Nonzygotic embryogenesis.- Embryogenic callus and suspension cultures from leaves of orchardgrass.- Somatic embryogenesis from seeds of melon.- Somatic embryogenesis from mature peanut seed.- Direct somatic embryogenesis from leaves and flower receptacles of cineraria.- Somatic embryogenesis from immature seeds of yellow-poplar.- Induction of somatic embryogenesis in conifers.- Use of protoplasts for plant improvement.- Isolation, culture, and fusion of tobacco and potato protoplasts.- Isolation of protoplasts from leaves of chrysanthemum and orchardgrass.- Haploid cultures.- Production of haploid tobacco plants using anther culture.- Transformation.- Transformation of tobacco and carrot using agrobacterium tumefaciens and expression of the b-glucuronidase (GUS) reporter gene.- Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens.- Construction and use of a simple gene gun for particle bombardment.- Germplasm preservation.- Vitrification as a method to cryopreserve shoot tips.- Secondary product expression in vitro.- Pigment production in Ajuga cell cultures.- In vitro plant pathology.- Variation in tissue culture.- Short-circuiting the fern life cycle: apospory in Ceratopteris richardii.- Commercial laboratory production.- Indexing for plant pathogens.- Culture indexing for bacterial and fungal contaminants.- List of some suppliers, addresses, and toll-free telephone numbers.
